OPTIMIZING THE PRODUCTION OF PECTINASE OF ORANGE PEEL WASTE BY PENICILLIUM CHRYSOGENUM MF318506 USING RESPONSE SURFACE METHODOLOGY IN SUBMERGED FERMENTATION

Pectinases find wide application in different industrial applications, e.g., textile, food, tea, paper and pulp, vegetable oil extractions, saccharification of agricultural remains, fermented drinks. Three fungal strains were screened for pectinase production. Results indicated that all are capable producing a enzyme. Penicillium chrysogenum MF318506 showed the highest activity (0.214 U/ml) on recommended medium at 30 ◦C 150 rpm, 6 days. Different wastes (orange peel, banana potato pomegranate wheat bran, rice bran) as carbon source. The maximum 0.48U/ml was obtained from Orange peel selected further studies. Screening important components production by Plackett-Burman design (PBD) 1.057 U/ml achieved with 3 % orange 2.0 g/l (NH4)2SO4, 6.0 peptone, KH2PO4, K2HPO4, 0.1 MgSO4.7H2O, 0.5 KCl FeSO4. Finally, Box-Behken (BBD) which uses response surface methodology (RSM) applied to optimization factors using PBD. 1.292U/ml observed 2.5% 4.5 peptone. Statistical optimization, enhanced 6.04 folds waste substrate. produced has potential applications fields.